{"id":4172,"date":"2026-08-16T05:00:56","date_gmt":"2026-08-16T05:00:56","guid":{"rendered":"https:\/\/climatesmartagrifood.ca\/?p=4172"},"modified":"2026-08-07T22:33:46","modified_gmt":"2026-08-07T22:33:46","slug":"research-roundup-dna-extraction-by-gg4ghg","status":"publish","type":"post","link":"https:\/\/climatesmartagrifood.ca\/fr\/research-roundup-dna-extraction-by-gg4ghg\/","title":{"rendered":"[Research Roundup] DNA Extraction Methods Shape Soil Microbial Community Profiles by GG4GHG"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"4172\" class=\"elementor elementor-4172\" data-elementor-post-type=\"post\">\n\t\t\t\t<div data-particle_enable=\"false\" data-particle-mobile-disabled=\"false\" class=\"elementor-element elementor-element-660dbe53 e-flex e-con-boxed e-con e-parent\" data-id=\"660dbe53\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5bc5426b elementor-widget elementor-widget-text-editor\" data-id=\"5bc5426b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>Studying soil microbial communities through DNA extraction can introduce methodological biases. What drives these biases, and what factors matter the most for accurately characterizing soil microbes? Read on to learn what researchers from the <strong><a href=\"https:\/\/grassland-genomics.usask.ca\/\" target=\"_blank\" rel=\"noopener\">GG4GHG team<\/a><\/strong> uncovered through a comparison of 12 field soils across Saskatchewan.<\/p><h3 aria-level=\"1\"><span data-contrast=\"none\">Keywords<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;335559738&quot;:360,&quot;335559739&quot;:80}\">\u00a0<\/span><\/h3><ul><li>DNA (Deoxyribonucleic acid)<\/li><li>Microbial communities \/ Microbiota<\/li><li>Primer biases<\/li><li>Soil extraction<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-172618c elementor-widget elementor-widget-image\" data-id=\"172618c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/drive.google.com\/file\/d\/1bSGRQXO_lzjS1b_xiDeB0VUDnKSp4Aq1\/view?usp=sharing\" target=\"_blank\" rel=\"noopener\">\n\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"800\" height=\"450\" src=\"https:\/\/climatesmartagrifood.ca\/wp-content\/uploads\/2026\/08\/GG4GHG-Cardoni-et-al-2026-1024x576.png\" class=\"attachment-large size-large wp-image-4277\" alt=\"\" srcset=\"https:\/\/climatesmartagrifood.ca\/wp-content\/uploads\/2026\/08\/GG4GHG-Cardoni-et-al-2026-1024x576.png 1024w, https:\/\/climatesmartagrifood.ca\/wp-content\/uploads\/2026\/08\/GG4GHG-Cardoni-et-al-2026-300x169.png 300w, https:\/\/climatesmartagrifood.ca\/wp-content\/uploads\/2026\/08\/GG4GHG-Cardoni-et-al-2026-768x432.png 768w, https:\/\/climatesmartagrifood.ca\/wp-content\/uploads\/2026\/08\/GG4GHG-Cardoni-et-al-2026-1536x864.png 1536w, https:\/\/climatesmartagrifood.ca\/wp-content\/uploads\/2026\/08\/GG4GHG-Cardoni-et-al-2026-18x10.png 18w, https:\/\/climatesmartagrifood.ca\/wp-content\/uploads\/2026\/08\/GG4GHG-Cardoni-et-al-2026.png 1920w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Click to view this visual abstract as a PDF<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b71ab37 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"b71ab37\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-74cf197 elementor-widget elementor-widget-text-editor\" data-id=\"74cf197\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<h3>Research Highlights<span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;335559738&quot;:360,&quot;335559739&quot;:80}\">\u00a0<\/span><\/h3><ul><li>DNA extraction is prone to biases by over- or under-representing microbial groups and affecting DNA quality<\/li><li>Accurate soil microbiome detection depends on both extraction and primer choice:<ul><li>Different combinations of methods performed better for different microbes<\/li><li>There is no one-size-fits-all method<\/li><li>Except Arbuscular Mycorrhizal Fungi (AMF), which showed resilience to extraction method biases<\/li><\/ul><\/li><li><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559685&quot;:720,&quot;335559737&quot;:0,&quot;335559738&quot;:0,&quot;335559739&quot;:160,&quot;335559740&quot;:279,&quot;335559991&quot;:360}\">Tailoring extraction and primer design strengthens the accuracy of soil microbiota analyses <\/span><\/li><\/ul><h3 aria-level=\"1\"><span data-contrast=\"none\">Editorial Reflections<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559685&quot;:0,&quot;335559737&quot;:0,&quot;335559738&quot;:360,&quot;335559739&quot;:80,&quot;335559740&quot;:279}\">\u00a0<\/span><\/h3><p>This study compares DNA extraction and primer choices across 12 Saskatchewan field soils with different soil and climate conditions, offering more realistic findings of how the methods perform. No single extraction-primer combination worked best for every sample, but one clear takeaway stands out: pairing an optimized extraction method with complementary primer sets leads to more complete and reliable soil microbiome analyses.<\/p><p>Le <strong><a href=\"https:\/\/grassland-genomics.usask.ca\/\" target=\"_blank\" rel=\"noopener\">GG4GHG project<\/a><\/strong>\u00a0aims to reduce greenhouse gases in the Canadian Prairies by identifying plant genotypes, species, and communities that contribute to ecosystem function. Using genomics, researchers study native plants and soil microbes to identify microbial groups that can improve soil carbon storage. A contributing author from the team is:<\/p><ul><li><a href=\"https:\/\/agbio.usask.ca\/faculty-and-staff\/people-pages\/jonathan-bennett.php\" target=\"_blank\" rel=\"noopener\">Jonathan Bennett<\/a> \u2013 Associate Professor, Department of Plant Sciences, University of Saskatchewan<\/li><\/ul><p><b><span data-contrast=\"auto\">Food for Thought:<\/span><\/b><span data-contrast=\"auto\"> Read the <a href=\"https:\/\/doi.org\/10.1155\/aess\/5563719\" target=\"_blank\" rel=\"noopener\">full article here<\/a>. In your view, what are the immediate and long-term implications of accurately characterizing soil microbial communities? As researchers work to identify microbial groups that support soil carbon storage, how promising is it to consider their potential role in advancing net-zero goals and contributing to broader climate solutions? <\/span><\/p><h3 aria-level=\"1\"><span data-contrast=\"none\">References<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;335559738&quot;:360,&quot;335559739&quot;:80}\">\u00a0<\/span><\/h3><p>Cardoni, M., Nguyen, H., Royle, J., &amp; Bennett, J. A. (2026). Lost in Extraction: Uncovering Bias in Soil Microbial Community Profiles. <i>Applied and Environmental Soil Science,<\/i> 2026(1), 5563719. <a href=\"https:\/\/doi.org\/10.1155\/aess\/5563719\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1155\/aess\/5563719<\/a>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bfd1d08 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"bfd1d08\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0abeafc elementor-widget elementor-widget-text-editor\" data-id=\"0abeafc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<h3 aria-level=\"1\"><span data-contrast=\"none\">About the Climate-Smart Agri-Food Network<\/span><span data-ccp-props=\"{&quot;134245418&quot;:true,&quot;134245529&quot;:true,&quot;335559738&quot;:360,&quot;335559739&quot;:80}\">\u00a0<\/span><\/h3><p><span data-contrast=\"none\">Funded by Genome Canada through the\u00a0<\/span><a href=\"https:\/\/genomecanada.ca\/challenge-areas\/climate-smart-agriculture-and-food-systems\/\" target=\"_blank\" rel=\"noopener\"><span data-contrast=\"none\">Climate\u2011Smart Agriculture and Food Systems<\/span><\/a><span data-contrast=\"none\">\u00a0portfolio, the Network brings together nine Interdisciplinary Challenge Teams (ICTs) and two hubs \u2014 the\u00a0<\/span><a href=\"https:\/\/climatesmartagrifood.ca\/fr\/ag-act-agricultural-genomics-action-centre\/\"><span data-contrast=\"none\">Agricultural Genomics Action Centre (AG\u2011ACt)<\/span><\/a><span data-contrast=\"none\">\u00a0and the\u00a0<\/span><a href=\"https:\/\/climatesmartagrifood.ca\/fr\/cs-dcc-climate-smart-data-collaboration-centre\/\"><span data-contrast=\"none\">Climate\u2011Smart Data Collaboration Centre (CS\u2011DCC)<\/span><\/a><span data-contrast=\"none\">\u00a0\u2014 to advance climate\u2011smart agriculture through genomic innovations.<\/span><span data-ccp-props=\"{}\">\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Studying soil microbial communities through DNA extraction can introduce methodological biases. What drives these biases, and what factors matter the most for accurately characterizing soil microbes? Read on to learn what researchers from the GG4GHG team uncovered through a comparison of 12 field soils across Saskatchewan. Keywords DNA (Deoxyribonucleic acid) Microbial communities \/ Microbiota Primer [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":4278,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[27,8],"tags":[],"class_list":["post-4172","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-homepage-news","category-news"],"_links":{"self":[{"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/posts\/4172","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/comments?post=4172"}],"version-history":[{"count":13,"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/posts\/4172\/revisions"}],"predecessor-version":[{"id":4290,"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/posts\/4172\/revisions\/4290"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/media\/4278"}],"wp:attachment":[{"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/media?parent=4172"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/categories?post=4172"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/climatesmartagrifood.ca\/fr\/wp-json\/wp\/v2\/tags?post=4172"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}